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cd31 pregnant human uterine smooth muscle cells  (PromoCell)


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    PromoCell cd31 pregnant human uterine smooth muscle cells
    Figure 3. Piezo1-meidiated Ca2+ influx in <t>CD31+</t> and CD31−human myometrial cells An intracellular calcium flux assay determined Piezo1 activity in phMEC (CD31+) and phUSMC (CD31−) cells. phMEC, phUSMC and HEK293 Piezo1KO cells were pre-treated with the Ca2+ indicator Calbryte (ex/em 493/515 nm) followed by exposure to Yoda1 (0.3 or 3 μM) ± the Piezo1 antagonist Dooku1 (10 μM) and the change in fluorescence (Cai2+) was measured. A, phMECs treated with 3 μM Yoda1 exhibited 4.09-fold increase in Ca2+ uptake (Cai2+) over 0.3 μM treated cells (P < 0.0001) which decreased by 35.74% when co-treated with Dooku1 (P = 0.0327) at 60 min. B, phUSMC experienced a 2.64-fold increase in fluorescence when challenged with 3 μM Yoda1 (P < 0.0001), with a respective decrease of 22.49% when co-treated with Dooku1 (P = 0.0326). C, Piezo1KO fluorescence did not vary significantly at any dose of Yoda1 or Yoda1 + Dooku1 relative to baseline (Kruskal–Wallis one-way ANOVA, P = 0.2622). D, left, ×10 bright field images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Right, Calbryte-induced fluorescence after 3 μM Yoda1 stimulation. ×20 fluorescence images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Data presented as ±SD.
    Cd31 Pregnant Human Uterine Smooth Muscle Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd31+pregnant+human+uterine+smooth+muscle+cells/10__1113_slash_jp283299-89-15-32?v=PromoCell
    Average 94 stars, based on 33 article reviews
    cd31 pregnant human uterine smooth muscle cells - by Bioz Stars, 2026-06
    94/100 stars

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    1) Product Images from "Novel identification and modulation of the mechanosensitive Piezo1 channel in human myometrium"

    Article Title: Novel identification and modulation of the mechanosensitive Piezo1 channel in human myometrium

    Journal: The Journal of Physiology

    doi: 10.1113/jp283299

    Figure 3. Piezo1-meidiated Ca2+ influx in CD31+ and CD31−human myometrial cells An intracellular calcium flux assay determined Piezo1 activity in phMEC (CD31+) and phUSMC (CD31−) cells. phMEC, phUSMC and HEK293 Piezo1KO cells were pre-treated with the Ca2+ indicator Calbryte (ex/em 493/515 nm) followed by exposure to Yoda1 (0.3 or 3 μM) ± the Piezo1 antagonist Dooku1 (10 μM) and the change in fluorescence (Cai2+) was measured. A, phMECs treated with 3 μM Yoda1 exhibited 4.09-fold increase in Ca2+ uptake (Cai2+) over 0.3 μM treated cells (P < 0.0001) which decreased by 35.74% when co-treated with Dooku1 (P = 0.0327) at 60 min. B, phUSMC experienced a 2.64-fold increase in fluorescence when challenged with 3 μM Yoda1 (P < 0.0001), with a respective decrease of 22.49% when co-treated with Dooku1 (P = 0.0326). C, Piezo1KO fluorescence did not vary significantly at any dose of Yoda1 or Yoda1 + Dooku1 relative to baseline (Kruskal–Wallis one-way ANOVA, P = 0.2622). D, left, ×10 bright field images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Right, Calbryte-induced fluorescence after 3 μM Yoda1 stimulation. ×20 fluorescence images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Data presented as ±SD.
    Figure Legend Snippet: Figure 3. Piezo1-meidiated Ca2+ influx in CD31+ and CD31−human myometrial cells An intracellular calcium flux assay determined Piezo1 activity in phMEC (CD31+) and phUSMC (CD31−) cells. phMEC, phUSMC and HEK293 Piezo1KO cells were pre-treated with the Ca2+ indicator Calbryte (ex/em 493/515 nm) followed by exposure to Yoda1 (0.3 or 3 μM) ± the Piezo1 antagonist Dooku1 (10 μM) and the change in fluorescence (Cai2+) was measured. A, phMECs treated with 3 μM Yoda1 exhibited 4.09-fold increase in Ca2+ uptake (Cai2+) over 0.3 μM treated cells (P < 0.0001) which decreased by 35.74% when co-treated with Dooku1 (P = 0.0327) at 60 min. B, phUSMC experienced a 2.64-fold increase in fluorescence when challenged with 3 μM Yoda1 (P < 0.0001), with a respective decrease of 22.49% when co-treated with Dooku1 (P = 0.0326). C, Piezo1KO fluorescence did not vary significantly at any dose of Yoda1 or Yoda1 + Dooku1 relative to baseline (Kruskal–Wallis one-way ANOVA, P = 0.2622). D, left, ×10 bright field images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Right, Calbryte-induced fluorescence after 3 μM Yoda1 stimulation. ×20 fluorescence images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Data presented as ±SD.

    Techniques Used: Calcium Flux Assay, Activity Assay, Fluorescence



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    cd31 pregnant human uterine smooth muscle cells  (PromoCell)
    94
    PromoCell cd31 pregnant human uterine smooth muscle cells
    Figure 3. Piezo1-meidiated Ca2+ influx in <t>CD31+</t> and CD31−human myometrial cells An intracellular calcium flux assay determined Piezo1 activity in phMEC (CD31+) and phUSMC (CD31−) cells. phMEC, phUSMC and HEK293 Piezo1KO cells were pre-treated with the Ca2+ indicator Calbryte (ex/em 493/515 nm) followed by exposure to Yoda1 (0.3 or 3 μM) ± the Piezo1 antagonist Dooku1 (10 μM) and the change in fluorescence (Cai2+) was measured. A, phMECs treated with 3 μM Yoda1 exhibited 4.09-fold increase in Ca2+ uptake (Cai2+) over 0.3 μM treated cells (P < 0.0001) which decreased by 35.74% when co-treated with Dooku1 (P = 0.0327) at 60 min. B, phUSMC experienced a 2.64-fold increase in fluorescence when challenged with 3 μM Yoda1 (P < 0.0001), with a respective decrease of 22.49% when co-treated with Dooku1 (P = 0.0326). C, Piezo1KO fluorescence did not vary significantly at any dose of Yoda1 or Yoda1 + Dooku1 relative to baseline (Kruskal–Wallis one-way ANOVA, P = 0.2622). D, left, ×10 bright field images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Right, Calbryte-induced fluorescence after 3 μM Yoda1 stimulation. ×20 fluorescence images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Data presented as ±SD.
    Cd31 Pregnant Human Uterine Smooth Muscle Cells, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd31+pregnant+human+uterine+smooth+muscle+cells/10__1113_slash_jp283299-89-15-32?v=PromoCell
    Average 94 stars, based on 1 article reviews
    cd31 pregnant human uterine smooth muscle cells - by Bioz Stars, 2026-06
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 3. Piezo1-meidiated Ca2+ influx in CD31+ and CD31−human myometrial cells An intracellular calcium flux assay determined Piezo1 activity in phMEC (CD31+) and phUSMC (CD31−) cells. phMEC, phUSMC and HEK293 Piezo1KO cells were pre-treated with the Ca2+ indicator Calbryte (ex/em 493/515 nm) followed by exposure to Yoda1 (0.3 or 3 μM) ± the Piezo1 antagonist Dooku1 (10 μM) and the change in fluorescence (Cai2+) was measured. A, phMECs treated with 3 μM Yoda1 exhibited 4.09-fold increase in Ca2+ uptake (Cai2+) over 0.3 μM treated cells (P < 0.0001) which decreased by 35.74% when co-treated with Dooku1 (P = 0.0327) at 60 min. B, phUSMC experienced a 2.64-fold increase in fluorescence when challenged with 3 μM Yoda1 (P < 0.0001), with a respective decrease of 22.49% when co-treated with Dooku1 (P = 0.0326). C, Piezo1KO fluorescence did not vary significantly at any dose of Yoda1 or Yoda1 + Dooku1 relative to baseline (Kruskal–Wallis one-way ANOVA, P = 0.2622). D, left, ×10 bright field images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Right, Calbryte-induced fluorescence after 3 μM Yoda1 stimulation. ×20 fluorescence images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Data presented as ±SD.

    Journal: The Journal of Physiology

    Article Title: Novel identification and modulation of the mechanosensitive Piezo1 channel in human myometrium

    doi: 10.1113/jp283299

    Figure Lengend Snippet: Figure 3. Piezo1-meidiated Ca2+ influx in CD31+ and CD31−human myometrial cells An intracellular calcium flux assay determined Piezo1 activity in phMEC (CD31+) and phUSMC (CD31−) cells. phMEC, phUSMC and HEK293 Piezo1KO cells were pre-treated with the Ca2+ indicator Calbryte (ex/em 493/515 nm) followed by exposure to Yoda1 (0.3 or 3 μM) ± the Piezo1 antagonist Dooku1 (10 μM) and the change in fluorescence (Cai2+) was measured. A, phMECs treated with 3 μM Yoda1 exhibited 4.09-fold increase in Ca2+ uptake (Cai2+) over 0.3 μM treated cells (P < 0.0001) which decreased by 35.74% when co-treated with Dooku1 (P = 0.0327) at 60 min. B, phUSMC experienced a 2.64-fold increase in fluorescence when challenged with 3 μM Yoda1 (P < 0.0001), with a respective decrease of 22.49% when co-treated with Dooku1 (P = 0.0326). C, Piezo1KO fluorescence did not vary significantly at any dose of Yoda1 or Yoda1 + Dooku1 relative to baseline (Kruskal–Wallis one-way ANOVA, P = 0.2622). D, left, ×10 bright field images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Right, Calbryte-induced fluorescence after 3 μM Yoda1 stimulation. ×20 fluorescence images of phMEC (top), phUSMC (middle), and Piezo1KO (bottom). Data presented as ±SD.

    Article Snippet: Cells captured by the beads were deemed CD31+ pregnant human myometrial endothelial cells (phMEC) and CD31− pregnant human uterine smooth muscle cells (phUSMC). phMECs cultured in endothelial basal medium 2 (C-22 011, PromoCell, Heidelberg, Germany) containing 10% fetal bovine serum (FBS) and 1% penicillin, while the phUSMC cells were cultured in DMEM with 50 U/ml streptomycin, 50 μg/ml penicillin, and 10% FBS and supplemented with oestrogen (15 ng/ml) and progesterone (200 ng/ml).

    Techniques: Calcium Flux Assay, Activity Assay, Fluorescence